Considerations for optimizing trapping conditions, such as flow rate and trapping volume, in nanoscale and microflow LC-MS.
On the impact of retentivity and characteristics of the stationary phases for reversed-phase proteomic separations and how you can use them to your advantage.
Can pairing column stationary phases improve nanoflow and microflow LC-MS results? Let’s take a closer look at the basics of trapping and the advantages and considerations for implementing a trapping step in a LC separation in part 1 of this 3-part series.
Q&A from webinar on using targeted 2D LC (RP-RP) for effective impurity analysis to address peak coelutions The Waters pharmacuetical team recently hosted a webinar discussion how two-dimentional LC can offer a solution to analyze… Read more >
Microflow or nanoflow chromatography has been used mostly in the academic and fundamental research environment in the past for its various advantages over analytical liquid chromatography, but rarely in the industrial settings. This is most… Read more >
What are matrix effects? In my last post, “What is Microflow,” I talked about two main parameters – better sampling efficiency and reduced matrix effects – for microflow LC/MS that result in higher sensitivity than its… Read more >
What are the differences between micro-scale and conventional LC? Microflow (a.k.a. microscale) LC has been widely applied to proteomic research for years. Recently, as microflow LC-MS systems continue to evolve, more and more laboratories in… Read more >
