During 2020, we collaborated with many of our customers to develop targeted liquid chromatography–mass spectrometry/mass spectrometry (LC-MS/MS) methods to detect SARS-CoV-2 (the virus that causes COVID-19) from biological samples. However, the combination of the biological… Read more >
A bowl of nuts, with polished or carved nutcrackers, is part of many traditional winter holiday feasts. Growing up in England in the 1970s, I enjoyed hazelnuts, almonds, walnuts and Brazil nuts as special treats… Read more >
The use of light during holidays or festivals – in any form, including candles, lanterns, or fireworks – is significant, as we celebrate and look ahead to the future. Many friends and colleagues recently celebrated… Read more >
A Breakthrough in Dioxin Analysis for Environmental Laboratories In September, the United States Environmental Protection Agency (USEPA) recognized the use of atmospheric pressure gas chromatography (APGC) with tandem quadrupole mass spectrometry (MS) as an acceptable… Read more >
From fundamental research to routine analysis, Waters’ latest innovations in mass spectrometry enable our customers to gain – and apply – insights across the scientific spectrum. Beginning with fundamental research where the focus is on… Read more >
Digging deep into the human biome, Professor Yoshiya Oda of the Graduate School of Medicine at the University of Tokyo looks to ion mobility technology to uncover deposits of insight It’s an established fact that… Read more >
The characterization of peptides and proteins can be challenging due to issues such as adsorption, varying hydrophobicities, and specificity to name just a few. When analyzing peptides by Liquid Chromatography (LC) and Mass Spectrometry (MS),… Read more >
What does “intelligence” mean in the context of biopharma analysis? Given the complex setting, much of the answer comes down to the task a user needs to complete, along with the question of who exactly… Read more >
There are two primary multi-attribute monitoring (MAM) assay choices for biologic development and QC: subunit protein mass analysis and peptide mapping by LC-MS. Here we explore how peptide mapping LC-MS MAM workflows are being used.
Two MAM approaches for biotherapeutic analysis are being implemented today; one focused on the analysis of monoclonal antibody (mAb) subunits, and the other focused on the analysis of peptides from a protein digest (peptide mapping workflow). Both have their advantages and disadvantages. Here, we explore MAM for mAb subunit analysis.
