New Preparative Chromatography Learning Tool: Purification Primer

By May 11, 2017

Beginners Guide to Preparative Liquid Chromatography

Purification, also known as preparative chromatography, continues to be an important part of pharmaceutical, fine chemical, natural product, and other laboratory workflows.

Chromatographers sometimes find themselves challenged as they move from analytical scale to preparative scales.  In an effort to help scientists overcome these challenges and gain a higher level of understanding and knowledge a new learning tool is now available. This primer, titled “Beginners Guide to Preparative Liquid Chromatography” provides both the novice as well as the experienced chromatographer a solid base of information along with many practical tips and techniques for successful purification chromatography.

Written by Waters scientist Catharine Layton, this primer addresses many topics including a general discussion of purification by liquid chromatography, best practices in method development, hardware and software considerations, and much more.

Chapters include:

  • Purification by liquid chromatography
  • Samples
  • Modes of separation
  • Quick start method development
  • Scouting
  • Alternative method development
  • Method scale-up
  • Prep calculator
  • System considerations
  • Sample introduction
  • Isolation of the purified product
  • Fraction analysis

As Catharine states in the introduction, “The intention of this primer is to provide liquid chromatographers with introductory information regarding the rapidly expanding field of LC purification. Fundamental principles such as the development of a chromatographic separation method, mathematical equations required for method scale-up, and commonly used modes for fraction collection are introduced. Reversed-phase is the primary mode of separation discussed since it is used in most preparative LC applications.”




Categories: Technologies