Get Empowered: Review Window and the Processing Method | Tip #98, Custom Fields
Tip #98: Creating Custom Fields in Empower (Part 16)
Creating Custom Fields in Empower:
- #82: The basic steps on creating a Custom Field
- #83: How to create a Custom Sample Set Field
- #84: How to create a Component Custom Field
- #85: How to create a Peak Custom Field
- #86: How to copy a Custom Field from one project to another
- #87: How to modify a Custom Field
- #88: How to create a Result Custom Field
- #89: How to modify a Custom Field that applies to specific samples and/or specific peaks
- #90: How to use Empower to evaluate whether the sample meets specific criteria
- #92: How to create one custom field to determine a sample pass/fail
- #93: How to create an Intersample Custom Field
- #94: How to create Intersample Custom Fields with a summary function
- #95: The difference between Summarize Custom Fields and Summarize Custom Fields Incrementally
- #96: How to report the pressure and temperature at a certain time point within a chromatogram
- #97: How to report the maximum pressure during a chromatographic run
This week, I want to share another great question I received.
Q: How do I use the average area of replicate injections of my sample to quantitate my analyte(s) of interest?
A: Normally, we quantitate the individual injections and then calculate the average amount; this is another way.
In this tip, I will show you how to use the average area of replicate injections of a sample to quantitate analyte(s) of interest (Part 16).
Let’s get started.
Create a Peak Type Custom Field, ‘data type real’ to calculate the average area of the replicate injections. This is an Intersample Custom Field with a summary function (figure 1).
The equation for a line is y=mx+b. In Empower, it is y=Bx+A. If we solve for ‘x’, we get x=(y-A)/B. In this case, we want to use the ‘average area’ which is calculated from the first Custom Field. I would recommend referencing the ‘last injection’ of the ‘last standard’ for A and B, so that you have the final coefficients (figure 2).
Now, we see the ‘average area’ for each component based on the duplicate injections of the sample and the ‘calculated amount’ based on that average (figure 3).
It’s that easy!
- This procedure can be followed using the QuickStart or Pro interface.
- Please refer to Tip #93 and Tip #94 for more information about Intersample Custom Fields.
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Next week in Empower Tip #99 – We’ll continue our series on Custom Fields.
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