Tip #58: Deriving Channels in Empower (Part 11)

Welcome back to Get Empowered! In our last tip-of-the-week blog for Empower Software, we reviewed Deriving Channels when working with 3D MS chromatography data in Empower.

• Tip 47: Smooth a chromatogram by Deriving a Channel (part 1)
• Tip 48: Correct for the time offset between detectors connected in series by Deriving a Channel (part 2)
• Tip 49: Subtract a baseline from a 2D chromatogram by Deriving a Channel (part 3).
• Tip 51: Subtract a 2D baseline from 3D PDA data by Deriving a Channel (part 4).
• Tip 52: Derive MaxPlot from 3D PDA data (part 5)
• Tip 53: Derive TotalPlot from 3D PDA data (part 6)
• Tip 54: Derive RatioPlot from 3D PDA data (part 7)
• Tip 55: Derive Timed Wavelength from 3D PDS data (part 8)
• Tip 56: Derive Single Mass from 3D MS data (part 9)
• Tip 57: Derive TIC plot from 3D MS data (part 10)

In this tip, we will continue the discussion on Deriving a TIC plot from 3D MS chromatography data; specifically, how to improve the noisy TIC plot.

You may recall the previous tip (Tip 57) when we derived the TIC Plot, it was noisy and I mentioned that it could be improved. The Component Detection Algorithm, or CODA, is a method that was specifically developed for LC/MS data in order to reduce random noise and high background so as to simplify interpretation of the data. CODA retains mass chromatograms that contain information (i.e., analyte peaks) in the data set and removes mass chromatograms that add noise to the TIC plot.

Let me show you how it is done.

Step 1

We begin by Deriving the TIC plot as we learned in Tip 57 (Figure 1).

Figure 1

Step 2

Return to the Method Set window, right-click on the Derived Channel in the tree and select Properties (Figure 2).

Figure 2

Step 3

Click the MS CODA tab, enable CODA, and click OK (Figure 3).

Figure 3

Step 4

Return to the Main Window, re-apply the Method Set, and notice the improvement in the TIC plot (Figure 4).

Figure 4

It’s that easy!

Final note: This procedure can be followed using the QuickStart or Pro interface.

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Next week in Empower Tip #59: Deriving Channels in Empower (part 12).

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