Get Empowered: Review Window and the Processing Method | Tip #42
Tip #42: Calculating impurities in samples (Part 3)
Welcome back to Get Empowered! In the last tip-of-the-week blog for Empower Software, we discussed how to calculate impurities in samples.
- Tip 40: Calculating unnamed impurities in samples (part 1)
- Tip 41: Calculating impurities in samples by % adjusted area (part 2)
This week we continue the discussion and look at another example (Part 3).
Previously, we wanted to express the impurity response as % adjusted area while excluding certain peaks such as a system peak from the calculation. In this example, we are going to express impurity response as % area against the main peak. We could create a custom field to make the calculation or we could use the features in Empower Software that are designed just for this purpose.
Let me show you how.
Step 1
Let’s start by bringing a sample set into Review, open the processing method, click the processing method tool, and click the Impurity tab (Figure 1).
Step 2
For the Impurity Response, select % Area Against Main Component from the drop-down list (Figure 2).
The calculation is (area of peak / area of main peak) x 100.
Step 3
Now let’s select the name of the main peak from the Main Component drop-down list (Figure 3).
Step 4
Return to the main window and click quantitate. The original %Area is next to the Impurity Response (Figure 4). (I did adjust the precision for the %Area field from 2 to 3.)
If we look at Impurity E the original %Area is 0.561% and now, compared to the main peak, it is 0.568% – which makes sense, since the area of the peak is now compared to the area of the main peak, which less than the total area for all peaks. (Hint – remember that you can see the total area by going to the Results window within Review and check the middle table called Chromatogram Result.)
It’s that easy!
I learned how to calculate impurities in samples in Empower! #EmpowerTips Click To TweetFinal note:
This procedure can be followed using the QuickStart or Pro interface.
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Next week in Empower Tip #43: Calculating Impurities (Part 4), Working with thresholds
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