Get Empowered: Review Window and the Processing Method | Tip #127, GPC/SEC Data
Tip #127: Working with GPC/SEC data in Empower Software (Part 4)
In this week’s tip, we are going to learn about doing universal calibration using Mark-Houwink constants (k and alpha). The advantages to this technique are: it is an easy and accurate method, you can use one calibration curve for different types of polymers and you can determine the intrinsic viscosity of a polymer. The disadvantage is, the accuracy depends on the accuracy of the Mark-Houwink constants.
Let’s get started.
Bring the Sample Set into the Alter Sample window and click the Amounts tool. Change the Sample Set Type to Standards & Unknowns (figure 1).
Click the K-Alphas tab. Enter the Ks and Alphas for the standards and samples. You can find these values in polymer handbooks and/or Google them (figure 2).
Create a Processing Method and on the Calibration tab, select Universal for the GPC Technique (figure 3).
After processing the Sample Set, we see the result for a sample. The calculation of Mv and [n]P (the intrinsic viscosity) are done in addition to the other molecular weight calculations (figure 4).
The calibration curve plots Retention Time on the x-axis versus Log(Mw[n]) on the y-axis to reflect the addition of the viscosity term (figure 5).
Click the Viscosity Law tab to view the Viscosity Law plot. This plot illustrates the relationship between viscosity and molecular weight. The slope of the plot is alpha, and the y-intercept is K (figure 6).
Here we view a comparison of the results for a polymer processed using the Universal calibration curve versus the Relative calibration curve developed in an earlier tip. Why is there a difference in the molecular weights (figure 7)? Please message me with the answer. (click to message)
It’s that easy!
- Intrinsic viscosity is the polymers contribution to the viscosity when it is in solution.
- This procedure can be followed using the QuickStart or Pro interface.
Please rate this Empower Tip of the Week
Next week in Empower Tip #128 – We will continue with the series on working with GPC/SEC data in Empower (Part 5).
- Get the latest Empower projects, options, and more. Check out the New Waters Marketplace.
- Want to learn more? Check out our Empower training courses and eLearning.
- Build your technical skills and professional network. Share, ask, and interact with peers from around the globe in dynamic discussions by joining our Informatics Community Forum.
- Empower is the first cloud-deployable, compliance-ready, enterprise chromatography data software – Learn more about Empower Cloud
Do you want Empower Tips sent to your inbox every week?
Questions? Tips of your own? Let us know!