Get Empowered: Empower User Question | Tip #204 Comparing UV Spectra from Different Channels in Empower (Part 2)

By December 16, 2020


Tip #204: Empower User Question: Comparing UV Spectra from Different Channels in a Report, at Various Retention Times

Welcome back to Get Empowered! In the last Empower tip-of-the-week post for Empower Chromatography Data System, we learned one of the ways to report spectra from different 3D PDA channels (Tip #203).

In last week’s tip, we answered a question from an Empower user regarding comparing spectra from different 3D PDA channels at the same retention time. In this week’s tip, I will show you how to report spectra from different 3D PDA channels which are extracted at various retention times.

The way to accomplish this goal is to build a library of UV spectra and then report the library (Refer to Tip #144, Creating a UV Library).

Let’s get started.

Step 1

Right click on the ‘Channels’ of data and select ‘Review’ (figure 1).

Figure 1

Figure 1

Step 2

Extract a chromatogram and either manually integrate the peaks of interest or use a processing method. From the ‘Spectrum Review’ menu, select ‘Keep Spectra When Switching Channels’ (figure 2).

Figure 2

Figure 2

Step 3

Go to the next ‘3D channel’, extract a chromatogram and integrate the peaks (figure 3).

Figure 3

Figure 3

Step 4

From the ‘Library’ menu, select ‘New Library’. Enter a name for the library and click ‘Create’ (figure 4).

Figure 4

Figure 4

Step 5

To add the spectra to the library, go to the ‘Library’ menu, select ‘Add to Library (library name)’ (figure 5).

Figure 5

Figure 5

Step 6

In the ‘Add Spectrum to Library’ dialogue, enter a name and description and click ‘OK’. Repeat for all spectra and click ‘OK’ (figure 6).

Figure 6

Figure 6

Now that we have a library, let’s build the Report Method.

Step 7

Go to the ‘Methods’ tab, right click and select ‘New Method’ > ‘Reporting Method’ (figure 7).

Figure 7

Figure 7

Step 8

When Report Publisher opens, cancel the ‘New Method/Group’ dialogue (figure 8).

Figure 8

Figure 8

Step 9

Expand ‘PDA/MS Groups’, expand ‘Spectral/Library Plots’ and double click on ‘Library Plot Group’ (figure 9).

Figure 9

Figure 9

Step 10

Open ‘Configuration Manager’, select ‘Libraries’ and size the window so it is on top of the ‘Report Publisher’. Click and drag the library from ‘Configuration Manager’ into the ‘Report Publisher’ (figure 10).

Figure 10

Figure 10

Step 11

When the ‘Open Report Method’ dialogue opens, select the last choice/option and click ‘OK’ (figure 11).

Figure 11

Figure 11

Step 12

Close Preview. Right click on the spectral plot and select ‘Spectral Plot Properties’ (figure 12).

Figure 12

Figure 12

Step 13

On the ‘Spectra’ tab, adjust the UV labels (figure 13).

Figure 13

Figure 13

Step 14

On the ‘Legend’ tab, expand UV Spectra and double click ‘Name’ (figure 14).

Figure 14

Figure 14

Step 15

The result is an overlay of the spectra with a legend under the plot (figure 15).

Figure 15

Figure 15

 

It’s that easy!

Final Note: You can use either the Pro or QuickStart interface for this tip.

 

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Next week in Empower Tip #205 – I will answer another Empower user question regarding reporting PDA data.

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Categories: Empower Tips