Get Empowered: Empower User Question | Tip #204 Comparing UV Spectra from Different Channels in Empower (Part 2)
Tip #204: Empower User Question: Comparing UV Spectra from Different Channels in a Report, at Various Retention Times
In last week’s tip, we answered a question from an Empower user regarding comparing spectra from different 3D PDA channels at the same retention time. In this week’s tip, I will show you how to report spectra from different 3D PDA channels which are extracted at various retention times.
The way to accomplish this goal is to build a library of UV spectra and then report the library (Refer to Tip #144, Creating a UV Library).
Let’s get started.
Right click on the ‘Channels’ of data and select ‘Review’ (figure 1).
Extract a chromatogram and either manually integrate the peaks of interest or use a processing method. From the ‘Spectrum Review’ menu, select ‘Keep Spectra When Switching Channels’ (figure 2).
Go to the next ‘3D channel’, extract a chromatogram and integrate the peaks (figure 3).
From the ‘Library’ menu, select ‘New Library’. Enter a name for the library and click ‘Create’ (figure 4).
To add the spectra to the library, go to the ‘Library’ menu, select ‘Add to Library (library name)’ (figure 5).
In the ‘Add Spectrum to Library’ dialogue, enter a name and description and click ‘OK’. Repeat for all spectra and click ‘OK’ (figure 6).
Now that we have a library, let’s build the Report Method.
Go to the ‘Methods’ tab, right click and select ‘New Method’ > ‘Reporting Method’ (figure 7).
When Report Publisher opens, cancel the ‘New Method/Group’ dialogue (figure 8).
Expand ‘PDA/MS Groups’, expand ‘Spectral/Library Plots’ and double click on ‘Library Plot Group’ (figure 9).
Open ‘Configuration Manager’, select ‘Libraries’ and size the window so it is on top of the ‘Report Publisher’. Click and drag the library from ‘Configuration Manager’ into the ‘Report Publisher’ (figure 10).
When the ‘Open Report Method’ dialogue opens, select the last choice/option and click ‘OK’ (figure 11).
Close Preview. Right click on the spectral plot and select ‘Spectral Plot Properties’ (figure 12).
On the ‘Spectra’ tab, adjust the UV labels (figure 13).
On the ‘Legend’ tab, expand UV Spectra and double click ‘Name’ (figure 14).
The result is an overlay of the spectra with a legend under the plot (figure 15).
It’s that easy!
Final Note: You can use either the Pro or QuickStart interface for this tip.
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Next week in Empower Tip #205 – I will answer another Empower user question regarding reporting PDA data.
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