Get Empowered: Empower User Question | Tip #201 Calculation of USP Signal-to-Noise Ratio in Empower

By November 25, 2020

Tip #201: Empower User Question: Calculation of USP Signal-to-Noise Ratio

Welcome back to Get Empowered! In the last Empower tip-of-the-week post for Empower Chromatography Data System, we answered an Empower user question about identifying peaks and celebrated a milestone with Empower Tip #200.

In this week’s tip, we will answer another question from an Empower user regarding the calculation of USP signal-to-noise ratio.

Q: Why am I not getting the USP signal-to-noise calculated when I use a ‘blank’ for the noise calculation? 

Another great question.

Let’s get started.

Please Note: For this tip, I am using generic data as the customers data is confidential.

Step 1

The result displayed does not have the USP signal-to-noise (s/n) nor the USP Noise calculated (figure 1).

Figure 1

Figure 1

Step 2

The ‘Suitability’ tab in the Processing Method shows the correct selection for calculating USP signal-to-noise (s/n) and the use of a blank injection to calculate noise (figure 2).

Figure 2

Figure 2

Step 3

Working backwards, ‘Alter Sample Set’ displays the ‘Sample Matrix’ field.  The selection for the ‘Blank’ is different from the rest of the samples and that is the problem (figure 3).

Figure 3

Figure 3

Step 4

Modify the ‘Sample Matrix’ to be the same for the blank (figure 4).

Figure 4

Figure 4

Step 5

Reprocess the Sample Set and now the result for the same sample shows the values are calculated (figure 5).

Figure 5

Figure 5

Step 6

Search on ‘Verifying System Performance’ in the online help and you will see a Tip regarding the Sample Matrix (figure 6).

Figure 6

Figure 6


It’s that easy!

Final Note:Refer to Tips #28-31 for more detail on calculating signal-to-noise (s/n) ratio


Please rate this Empower Tip of the Week

Next week in Empower Tip #202 – We will revisit our VLOG series showcasing Empower as an important tool to implement Method Lifecycle Management (MLCM) and discuss using the eCord with ACQUITY and ACQUITY Arc systems which automates column knowledge management and serves as a control strategy for column usage.


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Categories: Empower Tips