Tip #13: Entering a purity factor for our standards in the component editor

In the last Empower Tip of the Week for Empower Chromatography Data Software (CDS), we explored how to Save an Instrument Method with a Method Set.  This tip will explore how to enter a purity factor for our standards in the component editor.

When we purchase an analytical standard, the supplier provides a certificate of analysis which includes the purity of the standard.  We then need to incorporate that purity factor into Empower so that our calibration curve will be adjusted to reflect the purity.

Here is how it’s done.

Step 1

Click Browse Project in the navigation bar.  (figure 1)

Figure 1

Step 2

Click the Sample Sets tab, right click on a sample set and select Alter Sample.  (figure 2)

Figure 2

Step 3

Click the Amount tool. (figure 3)

Figure 3

Step 4

Click the Purity (Vial) tab. (figure 4)

Figure 4

Step 5

Enter the Purity for the component(s). (figure 5)  The allowable range is 0.001 – 150.000%.

Figure 5

Step 6

Click OK to the Component Editor. (figure 6)

Figure 6

Step 7

Save all changes in Alter Sample and exit out. (figure 7)

Figure 7

Step 8

Right click on the Sample Set and select Process.  Select the proper method set or processing method and click OK. (figure 8)

Figure 8

Step 9

Select the Result Sets tab, right click on the new Result Set and select Review. (figure 9)

Figure 9

Step 10

Click the in the tool bar and observe the X Value in the point table.  In the case of Acetone the amount entered for standard 1 was 937.500. With the purity of 99.0 entered, the amount used for the calibration curve is 928.125. (figure 10)

Figure 10

A final note:

1. You can enter the purity either post run in Alter Sample or prior to data acquisition by clicking the Components tool while in the Run Samples area and then follow steps 4-6 as described above.
   (figure 11)

Figure 11

Next week in Tip #14 – Interactive System Suitability

More resources:

• See all of our tips for using Empower CDS Software
• Mark your calendar to attend our annual Waters Informatics Users’ Conference in Tampa, Florida, May 15-18. More Information