Get Empowered: Tip #225 | Performing Glycan Analysis

By May 12, 2021


Tip #225: How to Perform Glycan Analysis and Generate GU Values in Empower

Welcome back to Get Empowered! In the last Empower tip-of-the-week blog for Empower Chromatography Data System, we learned about exporting a report with an Export Method (Tip #224).

In this week’s tip, we will learn how to perform Glycan analysis and generate GU values that can be used in the screening of Glycans. This example demonstrates the analysis with Glycans that has been labeled with a fluorescent tag. (Please Note: You must have the GPC option for your Empower system to use this procedure.)

Let’s get started.

Step 1

In the sample set, select ‘Inject Narrow Standards’ for the Dextran Ladder Standard and ‘Inject Broad Unknows’ for the Samples (figure 1).

empower

Figure 1

Step 2

With the row for the ‘Dextran Ladder Standard’ highlighted, click the ‘Amount’ tool, select ‘Molecular Weights’ tab for the current sample and enter the values for each peak in the Dextran Ladder. (Please Note: The number of values entered must match the number of integrated peaks in the chromatogram) (figure 2).

empower

Figure 2

Step 3

Create a GPC processing method, setting up peak detection and integration parameters for the narrow standard and the broad samples (figure 3).

empower

Figure 3

Step 4

On the Calibration tab, Select Relative for the GPC technique, 5th order fit type, time for the x-axis and low to high for the calibration order (figure 4).

empower

Figure 4

Step 5

Create a simple peak custom field which divides the peak molecular weight by 10000 and call it GU or GU Value (figure 5).

empower

Figure 5

Step 6

Process the Sample Set and view the result for the Dextran Ladder standard (figure 6).

empower

Figure 6

Step 7

View the resulting calibration curve (figure 7).

empower

Figure 7

Step 8

View the results for a sample (figure 8).

empower

Figure 8

 

It’s that easy!

Final Note: You can use either the Pro or QuickStart interface for this tip.

Please rate this Empower Tip of the Week


Next week in Empower Tip #226: We will answer an Empower user question regarding creating a View Filter to determine which Results have been Signed Off.

Empower

JOIN US for an Empower: Ask Me Anything Webinar on May 20, 2021

Neil will answer your questions about Processing Data. Learn More and Register.

More Empower Resources:

 

 

Do you want Empower Tips sent to your inbox every week?

Subscribe Now

Questions? Tips of your own? Let us know!

 


Categories: Empower Tips