Empower Tip #268 | Calculating Amount/Concentration

By March 9, 2022


Tip #268: Exploring parameters within the Sample Set Method that can affect the calculation of Amount/Concentration

Welcome back! In this week’s tip for Empower Chromatography Data System (CDS), we continue our series on calculating Amount/Concentration and will explore parameters within the Sample Set Method that can affect the calculation of Amount/Concentration. In last week’s tip, we discussed how Empower calculates Amount/Concentration, Tip #267.

The parameters we will explore are Purity, Sample Weight and Dilution.

Let’s get started.


Purity

Step 1

The Purity within the Component Editor is where you enter a purity factor for the standards. The amount entered on the ‘Value’ tab is multiplied by the ‘purity factor’ and those values will be used for the ‘x axis’ of the calibration curve (figure 1).

empower chromatography data system tip

figure 1

Step 2

Once the Sample Set has been processed, view the ‘x Values’ in the calibration curve point table. As an example, for ‘Standard 1’ the entered amount was ‘0.0400’ and the value used (once corrected for the purity value) is ‘0.0396’ (figure 2).

empower chromatography data system tip

figure 2

Step 3

The calculated amount in the sample is ‘0.099’ compared to ‘0.100’ which we saw in Tip #267 (figure 3).

empower chromatography data system tip

figure 3

Sample Weight and Dilution

Step 4

When working with standards, the amount entered in the Component Editor is multiplied by the ‘Sample Weight’ entered in the Sample Set Method and then divided by the ‘Dilution’, also entered in the Sample Set Method, and that final value is used for the ‘x axis’ of the calibration curve (figure 4).

empower chromatography data system tip

figure 4

Step 5

Once the Sample Set is processed, view the ‘x values’ in the calibration curve point table. For example, for ‘Standard 1’ the entered amount was ‘0.0400’ and the value used (once corrected for Purity, Sample Weight and Dilution) is ‘0.0792’ (figure 5).

empower chromatography data system tip

figure 5

Step 6

The calculated amount in the sample is now ‘0.198’ (figure 6)

empower chromatography data system tip

figure 6

Step 7

When working with samples the amount determined from the calibration curve is divided by the ‘Sample Weight’ entered in the Sample Set Method and then multiplied by the ‘Dilution’ entered in the Sample Set Method. That is the final value that Empower displays (figure 7).

empower chromatography data system tip

figure 7

Step 8

The calculated amount in the sample is now ‘19.824’ (figure 8).

empower chromatography data system tip

figure 8

It’s that easy!

Final Note: This can be done with either the Pro or QuickStart interface.

 

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Next time in Empower Tip #269– we will continue the series on how Empower calculates Amount/Concentration.

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Categories: Empower Tips