Why do we lose compounds while trying to measure them? Are they disappearing? Are they breaking down? Why and how? It all starts with adsorption and for certain metal-sensitive analytes, it renders chromatographic analysis using… Read more >
Late nights in the laboratory seem to be the only thing I can really count on when I am trying to get reproducible chromatography for certain compounds. But what I really want, is to go… Read more >
Retaining polar analytes using reversed phase (RP) chromatographic conditions have long frustrated scientists. Trying to promote interactions between polar analytes and a non-polar reversed phase surfaces can often bring the most experienced separation scientist to… Read more >
Many years ago, I was hired by a biopharma company to develop analytical methods for therapeutic oligonucleotides. When a new colleague warned me about sample loss on LC columns I looked at him with skepticism.… Read more >
In the midst of the COVID-19 pandemic the power of technology has emerged center stage, keeping the industry connected at a time when many ordinary avenues of communication have been cut off over safety concerns.… Read more >
Scientists from Waters and Pfizer collaborated to take on the challenge of engineering a novel reversed-phase LC column for high-throughput separations with enhanced resolution, selectivity, and recovery of target mAbs and ADCs.
We listened and learned how scientists separate mAbs and ADCs; then we designed a novel column for LC-MS bioseparations A critical step toward the prolific and successful use of monoclonal antibodies (mAb) as biotherapeutics occurred… Read more >
A new approach to stationary phase chemistry for reversed-phase LC analysis of proteins, including monoclonal antibodies and antibody drug conjugates, delivers higher fidelity data especially for MS-based peak identification.
Three tips to help you reduce physical contaminants from impacting your chromatographic column’s longevity.
Retaining and separating highly polar, water-soluble organic compounds has been a challenge for separations scientists who prefer reversed-phase chromatography as their technique of choice. See how Waters has addressed these analytical challenges with our T3 columns.