As chromatographers seek maximum separation performance on their liquid chromatography (LC) systems, a key factor is the use of LC columns with an optimally packed column bed density. Solid-core columns manufactured with strict quality control… Read more >
Waters’ new ACQUITY PREMIER Solution is designed to reduce variability associated with metal-sensitive analytes and eliminate time-consuming tasks like LC system and column passivation.
Why do we lose compounds while trying to measure them? Are they disappearing? Are they breaking down? Why and how? It all starts with adsorption and for certain metal-sensitive analytes, it renders chromatographic analysis using… Read more >
Late nights in the laboratory seem to be the only thing I can really count on when I am trying to get reproducible chromatography for certain compounds. But what I really want, is to go… Read more >
Retaining polar analytes using reversed phase (RP) chromatographic conditions have long frustrated scientists. Trying to promote interactions between polar analytes and a non-polar reversed phase surfaces can often bring the most experienced separation scientist to… Read more >
Many years ago, I was hired by a biopharma company to develop analytical methods for therapeutic oligonucleotides. When a new colleague warned me about sample loss on LC columns I looked at him with skepticism.… Read more >
In the midst of the COVID-19 pandemic the power of technology has emerged center stage, keeping the industry connected at a time when many ordinary avenues of communication have been cut off over safety concerns.… Read more >
Scientists from Waters and Pfizer collaborated to take on the challenge of engineering a novel reversed-phase LC column for high-throughput separations with enhanced resolution, selectivity, and recovery of target mAbs and ADCs.
We listened and learned how scientists separate mAbs and ADCs; then we designed a novel column for LC-MS bioseparations A critical step toward the prolific and successful use of monoclonal antibodies (mAb) as biotherapeutics occurred… Read more >
A new approach to stationary phase chemistry for reversed-phase LC analysis of proteins, including monoclonal antibodies and antibody drug conjugates, delivers higher fidelity data especially for MS-based peak identification.