Recent posts

Columns Matter, Too: Choosing Optimal Conditions for Trap-and-Elute Nanoflow and Microflow LC-MS

By May 14, 2018

Considerations for optimizing trapping conditions, such as flow rate and trapping volume, in nanoscale and microflow LC-MS.

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Columns Matter, Too: Choosing the Best Stationary Phase for NanoFlow and Microflow LC-MS

By April 3, 2018

On the impact of retentivity and characteristics of the stationary phases for reversed-phase proteomic separations and how you can use them to your advantage.

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Columns Matter, Too: How to Improve Nano and Microflow LC-MS Proteomics by Considering the Separation Itself

By March 22, 2018

Can pairing column stationary phases improve nanoflow and microflow LC-MS results? Let’s take a closer look at the basics of trapping and the advantages and considerations for implementing a trapping step in a LC separation in part 1 of this 3-part series.

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Webinar Highlights: Microfluidics for Macromolecules

By November 14, 2016

Hi, I’m Jim Murphy, Principal Consulting Chemist at Waters, and I recently collaborated with Lucinda (Cohen) Hittle to present an exciting BioanalysisZone webinar on the use of microflow chromatography for macromolecules. Lucinda, a Director of… Read more >

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Is using Microflow LC in an Industrial Setting Really that Cumbersome?

By August 30, 2016

Microflow or nanoflow chromatography has been used mostly in the academic and fundamental research environment in the past for its various advantages over analytical liquid chromatography, but rarely in the industrial settings. This is most… Read more >

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Microflow LC Offers Better Sensitivity Due to Reduced Matrix Effects!

By August 15, 2016

What are matrix effects? In my last post, “What is Microflow,”  I talked about two main parameters – better sampling efficiency and reduced matrix effects – for microflow LC/MS that result in higher sensitivity than its… Read more >

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What is microflow LC?

By July 28, 2016
Small Molecule Graph

What are the differences between micro-scale and conventional LC? Microflow (a.k.a. microscale) LC has been widely applied to proteomic research for years. Recently, as microflow LC-MS systems continue to evolve, more and more laboratories in… Read more >

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High-throughput Microscale Bioanalysis is not a Paradox

By May 21, 2016

  Question: When someone says microscale chromatography, what comes to mind? Increased sensitivity? Reduced sample volume? Enhanced MS sampling efficiency? Yes, yes, and yes! You might also say, “finicky,” “slow,” or “only one person in… Read more >

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Separations Science Guiding Disease Research at Colorado State University

By March 11, 2016

As infectious diseases go, tuberculosis (TB) is clearly one of the worst. Easily spread through the air by those with an active infection, it is a scourge that is decimating developing countries. The World Health… Read more >

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Microflow LC-MS in Early Discovery through Development

By April 23, 2015

Let’s face it. Microflow LC-MS is a challenge. Only a few do it well, and even fewer do it well consistently. And for those who have had success, their methods can completely fall apart when… Read more >

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