#63: About once a year our Empower Software product manager, Neil Lander, gets the following question: “Can I create a mirror image of my chromatogram?” The answer is yes, and it’s easy to do.
Removing the ability for individual users to either delete data or to disable audit trails is expected to be both implemented and validated for data integrity. But when changes are required, what editing tools should be accessible to an analyst?
#62: In Empower Software, deriving a channel lets us mathematically combine two channels of data. What if we wanted to combine more than two channels? There’s a relatively new feature that lets us combine up to 10 channels of data, called Formula Based Derived Channel. Let’s see how it works.
#61: Learn how to derive a channel for Timed Mass for 3D MS data in Empower Software. Timed Mass creates a channel that specifies a mass at specified starting times, which can maximize sensitivity and allow us to perform quantification for all components regardless of the differences in mass.
#60. Another option for Deriving Channels in Empower when working with 3D MS data is Total Plot, a 2D chromatogram that plots the sum of all ion intensities acquired across a specified mass range at every time point. It is a subset of the TIC plot, which we covered in Tip 57.
#59: We previously discussed MaxPlot for 3D PDA data in Tip 52. Today we are going to learn about MaxPlot for 3D MS data. MaxPlot is a 2D chromatogram that plots each time point at the maximum intensity across the mass range.
#58: In this tip, we will continue the discussion on deriving a total ion chromatogram (TIC) plot from 3D MS data; specifically, how to improve a noisy TIC plot.
#57: How to derive a channel in Empower Software when working with 3D mass spec (MS) data, or the Total Ion Chromatogram (TIC), which plots the sum of all ion intensities acquired across the mass range at every time point.
#56: If you have an ACQUITY QDa Mass Detector on your HPLC or UPLC instrument and collect 3D MS data, there are a number of options for deriving a channel. In today’s tip, we’ll discuss deriving Single Mass from 3D MS data.
#55: Have you ever had a sample where each component had a different lambda max and it was difficult to pick one wavelength to use for quantitation? We show how to use Timed Wavelength when Deriving Channels in Empower Software.