#66 This week we will begin a new multi-part discussion around using Method Sets in Empower Software. This post shows how to subtract a 3D baseline from 3D PDA data.
#65: In this final tip in our Deriving Channels series for using Empower Software, we learn about Switch To, a function that you can use to combine data from channels generated by different wavelengths or detectors into one, so that you process a single chromatogram.
#64: How do you look at the second derivative of a chromatogram, which can make it easier to identify coeluting peaks? Neil Lander tackles how to do it in Empower Software.
#63: About once a year our Empower Software product manager, Neil Lander, gets the following question: “Can I create a mirror image of my chromatogram?” The answer is yes, and it’s easy to do.
#62: In Empower Software, deriving a channel lets us mathematically combine two channels of data. What if we wanted to combine more than two channels? There’s a relatively new feature that lets us combine up to 10 channels of data, called Formula Based Derived Channel. Let’s see how it works.
#61: Learn how to derive a channel for Timed Mass for 3D MS data in Empower Software. Timed Mass creates a channel that specifies a mass at specified starting times, which can maximize sensitivity and allow us to perform quantification for all components regardless of the differences in mass.
#60. Another option for Deriving Channels in Empower when working with 3D MS data is Total Plot, a 2D chromatogram that plots the sum of all ion intensities acquired across a specified mass range at every time point. It is a subset of the TIC plot, which we covered in Tip 57.
#59: We previously discussed MaxPlot for 3D PDA data in Tip 52. Today we are going to learn about MaxPlot for 3D MS data. MaxPlot is a 2D chromatogram that plots each time point at the maximum intensity across the mass range.
#58: In this tip, we will continue the discussion on deriving a total ion chromatogram (TIC) plot from 3D MS data; specifically, how to improve a noisy TIC plot.
#57: How to derive a channel in Empower Software when working with 3D mass spec (MS) data, or the Total Ion Chromatogram (TIC), which plots the sum of all ion intensities acquired across the mass range at every time point.