#64: How do you look at the second derivative of a chromatogram, which can make it easier to identify coeluting peaks? Neil Lander tackles how to do it in Empower Software.
#63: About once a year our Empower Software product manager, Neil Lander, gets the following question: “Can I create a mirror image of my chromatogram?” The answer is yes, and it’s easy to do.
On the impact of retentivity and characteristics of the stationary phases for reversed-phase proteomic separations and how you can use them to your advantage.
Removing the ability for individual users to either delete data or to disable audit trails is expected to be both implemented and validated for data integrity. But when changes are required, what editing tools should be accessible to an analyst?
Q&A time: What is acrylamide? How dangerous is it? How do food testing laboratories detect it?
#62: In Empower Software, deriving a channel lets us mathematically combine two channels of data. What if we wanted to combine more than two channels? There’s a relatively new feature that lets us combine up to 10 channels of data, called Formula Based Derived Channel. Let’s see how it works.
Can pairing column stationary phases improve nanoflow and microflow LC-MS results? Let’s take a closer look at the basics of trapping and the advantages and considerations for implementing a trapping step in a LC separation in part 1 of this 3-part series.
#61: Learn how to derive a channel for Timed Mass for 3D MS data in Empower Software. Timed Mass creates a channel that specifies a mass at specified starting times, which can maximize sensitivity and allow us to perform quantification for all components regardless of the differences in mass.
Is your analytical method bound for the lab down the hall, or the lab halfway around the world? If you’re an analytical or QC scientist, get ready for more method transfers between organizations. We explore the complexities of analytical method transfer in part 2 of our ongoing series.
#60. Another option for Deriving Channels in Empower when working with 3D MS data is Total Plot, a 2D chromatogram that plots the sum of all ion intensities acquired across a specified mass range at every time point. It is a subset of the TIC plot, which we covered in Tip 57.