Get Empowered: Review Window and the Processing Method | Tip #61, Deriving Channels

By March 20, 2018


Tip #61: Deriving Channels in Empower (Part 14)

Welcome back to Get Empowered! In our last tip-of-the-week blog for Empower Software, we reviewed Deriving Channels when working with 3D MS chromatography data in Empower.

  • Tip 47: Smooth a chromatogram by Deriving a Channel (part 1)
  • Tip 48: Correct for the time offset between detectors connected in series by Deriving a Channel (part 2)
  • Tip 49: Subtract a baseline from a 2D chromatogram by Deriving a Channel (part 3).
  • Tip 51: Subtract a 2D baseline from 3D PDA data by Deriving a Channel (part 4).
  • Tip 52: Derive MaxPlot from 3D PDA data (part 5)
  • Tip 53: Derive TotalPlot from 3D PDA data (part 6)
  • Tip 54: Derive RatioPlot from 3D PDA data (part 7)
  • Tip 55: Derive Timed Wavelength from 3D PDS data (part 8)
  • Tip 56: Derive Single Mass from 3D MS data (part 9)
  • Tip 57: Derive TIC plot from 3D MS data (part 10)
  • Tip 58: Improve noisy TIC plot Derived from 3D MS data (part 11)
  • Tip 59: Derive MaxPlot from 3D MS data (part 12)
  • Tip 60: Derive Total Plot for 3D MS data (part 13)

In this tip, we will discuss another option for Deriving Channels when working with 3D mass spectrometry (MS) data, called Timed Mass.

You may recall that we learned about Timed Wavelength for 3D PDA data in Tip 55. Today we are going to learn about Timed Mass for 3D MS data. Timed Mass creates a channel that specifies a mass at specified starting times. This maximizes sensitivity and allows us to perform quantification for all components regardless of the differences in mass.

Let me show you how it is done.

Step 1

We begin by bringing a channel of 3D MS data into Review (Figure 1).

Empower Tips: 3D MS data into Review | Figure 1

Figure 1

Step 2

Go to the Method Set window and Derive a Channel, selecting MS: Timed Mass (Figure 2).

Empower Tip: Select MS: Timed Mass | Figure 2

Figure 2

Step 3

Enter the time and mass for each segment, and select the MS Scan (3D channel) from the Function drop-down list (Figure 3). The peak separation allows you to set a range for the desired mass as discussed in Tip 56.

Empower Tip: Enter time and mass for each segment/ Select the MS Scan | Figure 3

Figure 3

Step 4

We learned about using CODA in previous tips so let’s take advantage of that as well. Click the MS CODA tab, enable CODA, and click OK (Figure 4).

Empower Tip: Click the MS CODA tab/ Enable CODA | Figure 4

Figure 4

Step 5

Save the Derived Channel and select a Processing Method (Figure 5).

Empower Tip: Save the Derived Channel/Select a Processing Method | Figure 5

Figure 5

Step 6

Return to the Main Window and apply the Method Set. The Timed Mass chromatogram is displayed (Figure 6).

Empower Tip: Timed Mass Chromatogram is displayed | Figure 6

Figure 6

 

It’s that easy!

Final note: This procedure can be followed using the QuickStart or Pro interface.

 

Please rate this Empower Tip of the Week


 

Next week in Empower Tip #62: Deriving Channels in Empower (part 15).

 

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