Get Empowered: Review Window and the Processing Method | Tip #57, Deriving Channels

By February 22, 2018


Tip #57: Deriving Channels in Empower (Part 10)

Welcome back to Get Empowered! In our last tip-of-the-week blog for Empower Software, we reviewed Deriving Channels when working with 3D MS chromatography data in Empower..

  • Tip 47: Smooth a chromatogram by Deriving a Channel (part 1)
  • Tip 48: Correct for the time offset between detectors connected in series by Deriving a Channel (part 2)
  • Tip 49: Subtract a baseline from a 2D chromatogram by Deriving a Channel (part 3).
  • Tip 51: Subtract a 2D baseline from 3D PDA data by Deriving a Channel (part 4).
  • Tip 52: Derive MaxPlot from 3D PDA data (part 5)
  • Tip 53: Derive TotalPlot from 3D PDA data (part 6)
  • Tip 54: Derive RatioPlot from 3D PDA data (part 7)
  • Tip 55: Derive Timed Wavelength from 3D PDS data (part 8)
  • Tip 56: Derive Single Mass from 3D MS data (part 9)

In this tip, we will discuss another option for Deriving Channels when working with 3D MS chromatography data, called TIC plot.

You may hear your colleagues talking about TIC plots for 3D MS data. What is a TIC plot? A TIC, or Total Ion Chromatogram, is a 2D chromatogram that plots the sum of all ion intensities acquired across the mass range at every time point. The mass range is set in the instrument method. (There isn’t an analogous Derived Channel when working with 3D PDA data.)

Let me show you how it is done.

Step 1

We begin by bringing a channel of 3D MS data into Review (Figure 1).

Empower Tips: 3D MS data into Review | Figure 1

Figure 1

Step 2

Go to the Method Set window and Derive a Channel, selecting TIC (Figure 2).

Empower Tips: Derive a Channel, selecting TIC | Figure 2

Figure 2

Step 3

Select the MS Scan (3D channel) from the Function drop-down list (Figure 3).

Empower Tips: Select the MS Scan (3D channel) | Figure 3

Figure 3

Step 4

Save the Derived Channel and select a Processing Method (Figure 4).

Empower Tips: Save Derived Channel / select Processing Method | Figure 4

Figure 4

Step 5

Return to the main window and apply the Method Set. The Total Ion Chromatogram is displayed (Figure 5).

Empower Tips: Total Ion Chromatogram is displayed | Figure 5

Figure 5

The TIC plot appears to be a bit noisy. Can this be improved? Stay tuned for a future tip on improving the TIC plot by using CODA or Component Detection Algorithm.

It’s that easy!

Final note: This procedure can be followed using the QuickStart or Pro interface.

 

Please rate this Empower Tip of the Week


 

Next week in Empower Tip #58: Deriving Channels in Empower (part 11).

 

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