Get Empowered: Review Window and the Processing Method | Tip #22

By June 21, 2017


Tip #22: Setting the Peak Width and Threshold with the ApexTrack Algorithm

In the last Empower tip of the week blog for Empower Chromatography Software, we explored how to set the peak width and threshold when working with the traditional algorithm. This tip will address how to set the peak width and detection threshold when working with the ApexTrack peak detection/integration algorithm.

Peak Width and Detection Threshold work together for peak detection. Taking a minute to set these parameters will make creating the rest of the processing method easier. There is an auto peak width and auto threshold function in ApexTrack that makes it very easy to do.

Here’s how:

Step 1

Bring a channel of data into Review and from the View menu select Processing Method Layout (Figure 1).

Empower Tip: View Processing Method | Figure 1

Figure 1

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Step 2

The chromatogram and Processing Method are now side by side (Figure 2).

Empower Tip: Side by side view | Figure 2

Figure 2

Step 3

From the File menu select New > Processing Method (Figure 3).

Empower Tip: File menu, Processing method | Figure 3

Figure 3

Step 4

In the New Processing Method dialogue box, select ApexTrack for the Integration Algorithm (Figure 4).

Empower Tip: New processing method | Figure 4

Figure 4

Step 5

Click the Integration tab in the Processing Method and set the start and end time for peak detection (Figure 5).

Empower Tip: Integration tab | Figure 5

Figure 5

Step 6

Click the Integrate tool (figure 6).

Empower Tip: Integrate tool | Figure 6

Figure 6

Step 7

Empower calculates and displays the Result Peak Width (in seconds) and Result Detection Threshold (in microvolts) (Figure 7).

Empower Tip: Peak width and detection threshold | Figure 7

Figure 7

That is it. The peak width and detection threshold are now set.

Step 8

There are two ways to proceed as you create the Processing Method with the Apex Track algorithm:

  1. Empower can determine the optimum Peak Width and Detection Threshold for all data processed with this method. To follow this route, you don’t have to do anything further with Peak Width and Detection Threshold.
  2. Empower can use fixed values for the Peak Width and Detection Threshold for all data processed with this method. To follow this route, you can copy the Result Peak Width and Result Detection Threshold values into the new Processing Method. Right-click on the Result Peak Width value and select Copy to Processing Method (Figure 8). Repeat this for the Detection Threshold.
Empower Tip: Copying processing method | Figure 8

Figure 8

Step 9

Now the Peak Width and Detection Threshold values have been written into the Processing Method and these are the values Empower will use when you process data with this method (Figure 9).

Empower Tips: Processing method | Figure 9

Figure 9

Done. It’s that easy!

 

Final note:

  1. This works the same if you use Empower’s QuickStart Interface. The windows will be displayed differently with the chromatogram and processing method tiled horizontally.

 


Please rate this Empower Tip of the Week


 

 

Next week in Empower Tip #23: Interactively Adding Timed Events in the Chromatogram.

 

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